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Factors Affecting Enzyme Activity Class 11 Biology Notes | CBSE & NEET Factors Affecting Enzyme Activity ( NEET Note- Explanation)  Factors Affecting Enzyme Activity showing the effects of temperature, pH, substrate concentration, Vmax, and competitive inhibition for CBSE Class 11 and NEET Biology. -Dr.Sanjaykumar Pawar  What are Factors Affecting Enzyme Activity? Definition: Factors affecting enzyme activity are the conditions that can increase or decrease the speed of enzyme-catalysed reactions. Main Factors Temperature 🌡️ pH Substrate Concentration Inhibitors (Specific Chemicals) NEET Tip: Remember these four factors as "TPSI" T = Temperature P = pH S = Substrate concentration I = Inhibitors Line-by-Line Explanation Line 1 "The activity of an enzyme can be affected by a change in the conditions which can alter the tertiary structure of the protein." Easy Meaning Enzymes are proteins. Proteins have a special 3D (tertiary) struc...