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Prokaryotic Cells Explained for NEET | Easy Biology Notes

 Prokaryotic Cells Explained for NEET | Easy Biology Notes

Diagram of a prokaryotic bacterial cell showing cell wall, nucleoid DNA, plasmids, ribosomes, and mesosome structures in NEET biology style.
Labeled diagram of a prokaryotic cell showing nucleoid, plasmids, cell wall, and mesosome structure for NEET revision.


- Dr.Sanjaykumar Pawar 

1. Prokaryotes have a cell wall surrounding the cell membrane except in Mycoplasma.

  • Most prokaryotic cells (bacteria) have a cell wall outside the plasma membrane.
  • The cell wall gives shape and protection.
  • Exception: does not have a cell wall.

NEET Point: Mycoplasma is the smallest living cell and lacks a cell wall.


2. The semi-fluid matrix filling the cell is the cytoplasm.

  • The inside of the cell contains a jelly-like substance called cytoplasm.
  • It contains enzymes, nutrients, ribosomes, and other materials.
  • Many metabolic activities occur here.

3. There is no well-defined nucleus.

  • Prokaryotic cells do not have a true nucleus.
  • Their genetic material is not enclosed inside a nuclear membrane.

NEET Point: Prokaryotes have a nucleoid, not a true nucleus.


4. The genetic material is basically naked, not enveloped by a nuclear membrane.

  • DNA is directly present in the cytoplasm.
  • It is called naked DNA because no nuclear membrane surrounds it.

5. In addition to genomic DNA, many bacteria have small circular DNA outside the genomic DNA.

  • Bacteria usually have one main circular chromosome.
  • Apart from this, extra small circular DNA molecules may be present.

6. These smaller DNA are called plasmids.

  • Small circular DNA molecules are known as plasmids.
  • They replicate independently of the bacterial chromosome.

Definition: Plasmid = Extra-chromosomal circular DNA.


7. The plasmid DNA confers certain unique phenotypic characters.

  • Plasmids provide special characteristics to bacteria.
  • These characteristics help bacteria survive in different environments.

8. One such character is resistance to antibiotics.

  • Some plasmids carry genes that make bacteria resistant to antibiotics.
  • These genes are called R-genes (Resistance genes).

NEET Point: Antibiotic resistance is often due to plasmids.


9. Plasmid DNA is used to monitor bacterial transformation with foreign DNA.

  • Scientists use plasmids in genetic engineering.
  • Foreign genes can be inserted into plasmids.
  • Plasmids act as vectors to transfer genes into bacteria.

10. Nuclear membrane is found in eukaryotes.

  • A nuclear membrane surrounds the nucleus in eukaryotic cells.
  • It separates DNA from cytoplasm.

Examples of Eukaryotes:

  • Plants
  • Animals
  • Fungi
  • Protists

11. No organelles like those in eukaryotes are found in prokaryotes except ribosomes.

  • Prokaryotes lack membrane-bound organelles.

  • They do not have:

    • Mitochondria
    • Golgi apparatus
    • Endoplasmic reticulum
    • Lysosomes
    • Chloroplasts
  • Only ribosomes are present.

NEET Point: Prokaryotic ribosomes = 70S (50S + 30S)


12. Prokaryotes have something unique in the form of inclusions.

  • Prokaryotic cells contain inclusion bodies.
  • They store reserve materials.

Examples:

  • Glycogen granules
  • Phosphate granules
  • Sulphur granules

13. Mesosome is a specialized differentiated form of cell membrane.

  • Mesosomes are special structures found in prokaryotes.
  • They are formed by folding of the plasma membrane inward.

14. They are essentially infoldings of cell membrane.

  • Mesosomes are inward projections of the plasma membrane.
  • They increase membrane surface area.

Cell Envelope and its Modifications

15. Most bacterial cells have a chemically complex cell envelope.

  • The outer covering of bacteria is called the cell envelope.
  • It protects the cell from damage.

16. The cell envelope consists of three layers.

Layers of Cell Envelope (Outside → Inside)

  1. Glycocalyx
  2. Cell Wall
  3. Plasma Membrane

NEET Diagram Order: Glycocalyx → Cell Wall → Plasma Membrane


17. These layers work together as a protective unit.

  • Each layer has its own function.
  • Together they protect the bacterial cell.

Gram Positive and Gram Negative Bacteria

18. Bacteria are classified based on Gram staining.

  • A staining technique developed by is called Gram staining.
  • It differentiates bacteria based on cell wall structure.

19. Gram-positive bacteria

  • Retain the violet stain.
  • Appear purple under microscope.

Examples:

  • Bacillus
  • Clostridium

20. Gram-negative bacteria

  • Do not retain violet stain.
  • Appear pink/red after counterstaining.

Examples:

  • Escherichia coli
  • Salmonella

Glycocalyx

21. Glycocalyx differs in composition and thickness.

  • Different bacteria have different glycocalyx structures.
  • Thickness and chemical composition vary.

22. Loose sheath is called slime layer.

  • In some bacteria, glycocalyx is thin and loose.
  • It is called the slime layer.

Function: Protection and attachment to surfaces.


23. Thick and tough glycocalyx is called capsule.

  • Some bacteria possess a thick glycocalyx.
  • This thick covering is called a capsule.

Function: Protects bacteria from host immune system.


Cell Wall

24. Cell wall determines cell shape.

  • It gives definite shape to bacteria.
  • Prevents deformation.

25. Cell wall provides strong structural support.

  • It protects the bacterial cell from mechanical injury.

26. Prevents bursting or collapsing.

  • Prevents osmotic bursting when water enters the cell.
  • Maintains cell integrity.

Plasma Membrane

27. Plasma membrane is selectively permeable.

  • Allows some substances to enter and leave.
  • Blocks unwanted substances.

28. It interacts with the outside world.

  • Exchange of nutrients, gases, and wastes occurs through it.

29. Structure is similar to eukaryotic plasma membrane.

  • Both follow the fluid mosaic model.
  • Made mainly of phospholipids and proteins.

Mesosome

30. Mesosome is formed by extensions of plasma membrane.

  • Plasma membrane folds inward and forms mesosomes.

31. These extensions occur as:

  • Vesicles
  • Tubules
  • Lamellae

32. Functions of Mesosomes

  • Help in cell wall formation.
  • Assist DNA replication.
  • Help chromosome segregation during cell division.
  • Aid respiration.
  • Increase surface area of plasma membrane.
  • Help secretion processes.

Quick NEET Revision Table

Structure Important Function
Cell Wall Shape and protection
Cytoplasm Site of metabolic reactions
Nucleoid Contains naked DNA
Plasmid Antibiotic resistance, genetic engineering
Ribosome (70S) Protein synthesis
Inclusion Bodies Storage of reserve food
Glycocalyx Protection and adhesion
Capsule Prevents phagocytosis
Slime Layer Attachment and protection
Plasma Membrane Selective transport
Mesosome Respiration, cell wall formation, DNA replication

NEET One-Liners

  1. Prokaryotes lack a true nucleus.
  2. DNA is naked and circular.
  3. Plasmids are extra-chromosomal circular DNA.
  4. Prokaryotes contain 70S ribosomes.
  5. Mycoplasma lacks a cell wall.
  6. Cell envelope = Glycocalyx + Cell Wall + Plasma Membrane.
  7. Glycocalyx may form slime layer or capsule.
  8. Mesosomes are infoldings of plasma membrane.
  9. Gram-positive bacteria retain Gram stain.
  10. Inclusion bodies store reserve materials. 

Below are CBSE Class 11 Biology Exam-Oriented Questions and Answers from the topic "Prokaryotic Cell Structure, Cell Envelope and Mesosomes".


1. Multiple Choice Questions (MCQs)

Q1. Which organism lacks a cell wall?

a) Bacillus
b) E. coli
c) Mycoplasma
d) Cyanobacteria

Answer: c) Mycoplasma


Q2. The genetic material in prokaryotes is:

a) Enclosed in nucleus b) Naked DNA c) Linear DNA d) Histone-bound DNA

Answer: b) Naked DNA


Q3. Extra-chromosomal circular DNA in bacteria is called:

a) Chromosome b) Nucleoid c) Plasmid d) Mesosome

Answer: c) Plasmid


Q4. Ribosomes present in prokaryotes are:

a) 80S b) 70S c) 60S d) 50S

Answer: b) 70S


Q5. The outermost layer of bacterial cell envelope is:

a) Plasma membrane b) Cell wall c) Glycocalyx d) Mesosome

Answer: c) Glycocalyx


Q6. Thick and tough glycocalyx is called:

a) Slime layer b) Capsule c) Cell wall d) Lamella

Answer: b) Capsule


Q7. Mesosomes are formed by:

a) Cell wall b) Ribosomes c) Plasma membrane d) Cytoplasm

Answer: c) Plasma membrane


Q8. Gram-positive bacteria:

a) Do not retain Gram stain b) Retain Gram stain c) Lack cell wall d) Lack DNA

Answer: b) Retain Gram stain


2. Very Short Answer Questions (1 Mark)

Q1. What is a nucleoid?

Answer: The region containing naked DNA in a prokaryotic cell is called nucleoid.


Q2. Name the cell organelle present in prokaryotes.

Answer: Ribosomes.


Q3. What are plasmids?

Answer: Small circular extra-chromosomal DNA molecules present in bacteria.


Q4. Name the smallest living cell.

Answer: Mycoplasma.


Q5. What is glycocalyx?

Answer: The outermost covering of bacterial cell envelope.


Q6. Name two forms of glycocalyx.

Answer: Slime layer and capsule.


Q7. What is the function of cell wall?

Answer: It provides shape and protection.


Q8. What is mesosome?

Answer: Infolding of plasma membrane in prokaryotes.


3. Short Answer Questions (2–3 Marks)

Q1. Write any three characteristics of prokaryotic cells.

Answer:

  1. Lack true nucleus.
  2. Possess naked circular DNA.
  3. Lack membrane-bound organelles.

Q2. What are plasmids? Mention one importance.

Answer: Plasmids are small circular DNA molecules present outside bacterial chromosome.

Importance: They often carry antibiotic resistance genes.


Q3. Differentiate between capsule and slime layer.

Capsule Slime Layer
Thick and tough Thin and loose
Well organized Unorganized
Protects from phagocytosis Helps in attachment

Q4. Mention any three functions of mesosomes.

Answer:

  1. Cell wall formation.
  2. DNA replication.
  3. Respiration.

Q5. What is the cell envelope?

Answer: The cell envelope is the protective covering of bacterial cells consisting of:

  • Glycocalyx
  • Cell wall
  • Plasma membrane

4. Long Answer Questions (5 Marks)

Q1. Describe the structure of a typical prokaryotic cell.

Answer:

A typical prokaryotic cell consists of:

  1. Cell Envelope

    • Glycocalyx
    • Cell wall
    • Plasma membrane
  2. Cytoplasm

    • Semi-fluid matrix containing enzymes and ribosomes.
  3. Genetic Material

    • Naked circular DNA present in nucleoid.
  4. Plasmids

    • Extra-chromosomal circular DNA.
  5. Ribosomes

    • 70S ribosomes for protein synthesis.
  6. Mesosomes

    • Infoldings of plasma membrane helping in respiration and DNA replication.
  7. Inclusion Bodies

    • Storage granules for reserve food.

Q2. Explain the structure and functions of bacterial cell envelope.

Answer:

The bacterial cell envelope has three layers:

1. Glycocalyx

  • Outermost layer.
  • May form slime layer or capsule.

2. Cell Wall

  • Provides shape.
  • Prevents bursting and collapsing.

3. Plasma Membrane

  • Selectively permeable.
  • Regulates movement of substances.

Functions:

  • Protection
  • Structural support
  • Selective transport
  • Prevention of osmotic damage

5. Assertion and Reason Questions

Q1.

Assertion (A): Prokaryotes lack a true nucleus.

Reason (R): Their DNA is not enclosed by a nuclear membrane.

a) Both A and R are true and R is correct explanation of A.
b) Both A and R are true but R is not correct explanation.
c) A is true but R is false.
d) A is false but R is true.

Answer: a)


Q2.

Assertion (A): Plasmids are important in genetic engineering.

Reason (R): They can carry foreign DNA into bacteria.

Answer: a)


Q3.

Assertion (A): Mesosomes are formed by plasma membrane.

Reason (R): Mesosomes help in cell wall formation and respiration.

Answer: a)


6. Fill in the Blanks

  1. The DNA of prokaryotes is __________. Answer: Naked

  2. Extra-chromosomal DNA is called __________. Answer: Plasmid

  3. Prokaryotic ribosomes are __________ type. Answer: 70S

  4. The outermost layer of bacterial cell envelope is __________. Answer: Glycocalyx

  5. Thick glycocalyx is called __________. Answer: Capsule

  6. Loose glycocalyx is called __________. Answer: Slime layer

  7. Mesosomes are infoldings of __________ membrane. Answer: Plasma

  8. The DNA-containing region of bacteria is called __________. Answer: Nucleoid


7. Match the Columns

Question

Column A Column B
A. Plasmid 1. Protein synthesis
B. Ribosome 2. Antibiotic resistance
C. Capsule 3. Protection
D. Mesosome 4. Respiration

Answer

A → 2
B → 1
C → 3
D → 4


8. Statement-Based Questions

Q1. Read the statements carefully:

I. Prokaryotes have a true nucleus.
II. Prokaryotes possess 70S ribosomes.

Choose the correct option:

a) Both statements are true
b) Both statements are false
c) I is false but II is true
d) I is true but II is false

Answer: c)


Q2.

I. Plasmids are extra-chromosomal DNA.
II. Plasmids are useful in genetic engineering.

a) Both true
b) Both false
c) Only I true
d) Only II true

Answer: a)


9. Case Study Questions

Case Study 1

A scientist isolated a bacterium containing a circular chromosome and small circular DNA molecules. The bacterium had a capsule and 70S ribosomes.

Questions

Q1. What are the small circular DNA molecules called?

Answer: Plasmids

Q2. What is the function of plasmids?

Answer: They carry special traits such as antibiotic resistance.

Q3. Which type of ribosomes are present?

Answer: 70S ribosomes

Q4. What is the function of capsule?

Answer: Protection from adverse conditions and phagocytosis.


Case Study 2

A bacterial cell possesses glycocalyx, cell wall and plasma membrane. It also contains mesosomes.

Questions

Q1. Name the three layers of cell envelope.

Answer:

  • Glycocalyx
  • Cell wall
  • Plasma membrane

Q2. Which structure regulates movement of substances?

Answer: Plasma membrane

Q3. What are mesosomes?

Answer: Infoldings of plasma membrane.

Q4. Mention one function of mesosomes.

Answer: Respiration or DNA replication.


Important CBSE Board Questions

Frequently Asked 1-Mark Questions

  • Define plasmid.
  • What is nucleoid?
  • Name the smallest living cell.
  • What is glycocalyx?
  • What are inclusion bodies?

Frequently Asked 3-Mark Questions

  • Explain plasmids and their significance.
  • Differentiate capsule and slime layer.
  • Write functions of mesosomes.

Frequently Asked 5-Mark Questions

  • Describe the structure of a prokaryotic cell.
  • Explain bacterial cell envelope with diagram.
  • Differentiate Gram-positive and Gram-negative bacteria. 
🔷  INTERNAL LINKS 
/biology/cell-structure-prokaryotic-vs-eukaryotic
/biology/plasmid-definition-and-functions
/biology/mesosome-structure-and-functions
/biology/bacterial-cell-envelope
/neet/biology-important-topics
/neet/biology-revision-notes
/biology/gram-positive-vs-gram-negative-bacteria
/biology/mycoplasma-characteristics




Prokaryotic Cell Mind Map
PROKARYOTIC CELL (NEET MIND MAP)
1. Cell Envelope
Glycocalyx
- Slime layer (loose, protective)
- Capsule (thick, virulence factor)
Cell Wall
- Gives shape
- Prevents bursting
- Structural support
- Basis of Gram + / Gram - classification
Plasma Membrane
- Selectively permeable
- Controls transport
- Similar to eukaryotes
2. Cytoplasm
- Semi-fluid matrix
- Site of metabolic reactions
3. Genetic Material
Nucleoid
- No nuclear membrane
- Naked circular DNA (single chromosome)
Plasmid
- Extra-chromosomal DNA
- Antibiotic resistance genes
- Used in genetic engineering
4. Cell Organelles (Absent)
- No mitochondria
- No ER
- No Golgi apparatus
- No membrane-bound organelles
- Only 70S ribosomes present
5. Inclusion Bodies
- Storage granules
- Glycogen, phosphate, sulfur
6. Mesosome
- Infoldings of plasma membrane
- Types: vesicles, tubules, lamellae
- Functions:
• Respiration
• DNA replication
• Cell division
• Cell wall formation
7. Special Case: Mycoplasma
- No cell wall
- Smallest living cell
8. Gram Classification
Gram Positive: Thick peptidoglycan wall
Gram Negative: Thin wall + outer membrane

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